crispr-gene-editor

What is this?

🎯 Simulator Tips

📚 Glossary

CRISPR

Clustered Regularly Interspaced Short Palindromic Repeats

Cas9

CRISPR-associated protein 9 - the cutting enzyme

GuideRNA

RNA that directs Cas9 to target DNA sequence

PAM

Protospacer Adjacent Motif - required sequence for Cas9 binding

DoubleStrandBreak

Cut through both DNA strands

NHEJ

Non-Homologous End Joining - error-prone DNA repair

HDR

Homology-Directed Repair - precise repair using template

OffTarget

Unintended cuts at wrong genome locations

Knockout

Disabling a gene

Knockin

Inserting new DNA

SomaticEditing

Editing body cells (not inherited)

GermlineEditing

Editing reproductive cells (inherited)

🏆 Key Figures

Jennifer Doudna

Co-developed CRISPR-Cas9 for genome editing

Emmanuelle Charpentier

Co-developed CRISPR-Cas9

Feng Zhang

First to use CRISPR in human cells

David Liu

Invented base editing and prime editing

Francisco Mojica

Discovered CRISPR sequences in bacteria

💬 Message to Learners

{'encouragement': "You're learning about technology that won the Nobel Prize and is already curing genetic diseases. CRISPR is one of the most important discoveries of our time.", 'reminder': 'With great power comes great responsibility. Gene editing can cure disease, but must be used wisely.', 'action': 'Design a guide RNA in the simulator. See how precise gene editing works. Imagine the diseases you could cure.', 'dream': 'A scientist in Kenya might cure sickle cell disease that affects millions in Africa. A researcher in India might develop disease-resistant crops. This technology is for everyone.', 'wiaVision': 'WIA Pin Code brings Nobel Prize-winning biotechnology to everyone. The power to edit genes is now accessible. Learn it. Use it responsibly. Change the world.'}